Nano-sized metals have been introduced as a promising solution for microbial resistance to\nantimicrobial agents. Silver nanoparticles (AgNPs) have been proven to possess good antimicrobial\nactivity. Green synthesis of AgNPs has been reported as safe, low cost and ecofriendly. This\nmethodology uses extracts originating from different plants to reduce silver ions from AgNO3 into\nnano-sized particles. In this study, extracts of several plants including ginger, garlic, capsicum and\ntheir mixtures were successfully used to produce AgNPs. Numerous spectroscopic, light scattering\nand microscopic techniques were employed to characterize the synthesized AgNPs. Agar well\ndiffusion assay was performed to investigate the antimicrobial activity of AgNPs. The biosynthesized\nAgNPs have spherical shape with a size range of 20-70 nm. Garlic extract, pure or in mixture with\nginger extract, generated AgNPs of the smallest size. The presence of the plant-origin capping\nagents surrounding AgNPs was proven by Fourier-transform infrared spectroscopy. The AgNPs, at a\nconcentration of 50 microg/mL, demonstrated potent antimicrobial activity against Staphyloccocus aureus,\nEscherichia coli and Candida albicans as indicated by the zones of inhibitions. Our results revealed that\nAgNPs having potent antimicrobial activity could be prepared using different pure plant extracts and\ntheir mixtures.
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